LOCUS       Exported               12045 bp DNA     circular SYN 06-AUG-2025
DEFINITION  Gateway binary vector pGWB511 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S.
  TITLE     Improved Gateway Binary Vectors: High-Performance Vectors for 
            Creation of Fusion Constructs in Transgenic Analysis of Plants
  JOURNAL   Biosci. Biotechnol. Biochem. (2007) In press
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   Nakagawa T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, 
            Center for Integrated Research in Science; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, 
            Fax:81-852-32-6109)
REFERENCE   3  (bases 1 to 12045)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12045)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 12045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci.
            Biotechnol. Biochem. (2007) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, Center for
            Integrated Research in Science"; pages: "81-852-32-6109"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1318..1333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1356..1386
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1440..2096
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2441..2743
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2787..2911)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2922..2945
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     terminator      2967..3219
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3253..3268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3276..3292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3300..3330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3345..3366)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3402..3654)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3808..4830)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4882..5061)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5567..5591)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6112..6900
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7149..7737
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7923..8063)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8407..8601)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8670..9740)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10172..10798)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggcct
     1321 cgaggcctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     1381 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     1441 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1501 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1561 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1621 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1681 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1741 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1801 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1861 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1921 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1981 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2041 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2101 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2161 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2221 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2281 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     2341 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     2401 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     2461 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     2521 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     2581 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     2641 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2701 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2761 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2881 tttctcgttc agctttcttg tacaaagtgg ttgataacag cgactacaag gatgacgatg
     2941 acaaggctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     3001 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     3061 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     3121 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     3181 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa
     3241 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3361 acattaggct gaattaggcg cgcctatttc tattaattcc cgatctagta acatagatga
     3421 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     3481 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     3541 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     3601 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
     3661 attcggggga tcgatcccgc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt
     3721 tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca
     3781 gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg
     3841 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     3901 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     3961 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     4021 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     4081 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     4141 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     4201 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     4261 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     4321 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     4381 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     4441 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     4501 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     4561 gcaataggtc aggctctcgc tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc
     4621 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     4681 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     4741 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
     4801 agacgtcgcg gtgagttcag gctttttcat atcttattgc cccccgggat cagatctgga
     4861 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     4921 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     4981 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     5041 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     5101 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     5161 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     5221 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     5281 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     5341 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     5401 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     5461 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     5521 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     5581 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     5641 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     5701 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     5761 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     5821 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     5881 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     5941 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     6001 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     6061 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     6121 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     6181 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     6241 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     6301 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     6361 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     6421 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     6481 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     6541 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     6601 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     6661 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     6721 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     6781 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     6841 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     6901 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     6961 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     7021 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     7081 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7141 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7201 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7261 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7321 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7381 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7441 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7501 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7561 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7621 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7681 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7741 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7801 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7861 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7921 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     7981 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     8041 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     8101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     8221 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     8281 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     8341 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     8401 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     8461 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     8521 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     8581 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     8641 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     8701 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     8761 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     8821 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     8881 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     8941 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     9001 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     9061 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     9121 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     9181 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
     9241 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
     9301 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
     9361 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
     9421 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
     9481 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
     9541 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
     9601 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
     9661 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
     9721 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     9781 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     9841 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     9901 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     9961 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    10021 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    10081 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    10141 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    10201 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    10261 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    10321 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    10381 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    10441 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    10501 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    10561 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    10621 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    10681 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    10741 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    10801 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    10861 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    10921 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    10981 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    11041 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    11101 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    11161 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    11221 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    11281 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    11341 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    11401 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    11461 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    11521 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    11581 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    11641 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    11701 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    11761 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    11821 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    11881 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    11941 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    12001 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
